Rubisco in complex with Rubisco large subunit methyltransferase.

Rubisco small and large subunit N-methyltransferases. Bi- and mono-functional methyltransferases that methylate the small and large subunits of Rubisco.

Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco)is methylated at the alpha-amino group of the N-terminal methionine of the processed form of the small subunit (SS), and at the epsilon-amino group of lysine-14 of the large subunit (LS) in some species. The Rubisco LS methyltransferase (LSMT) gene has been cloned and expressed from pea and specifically methylates lysine-14 of the LS of R...

Structure of Rubisco from Arabidopsis thaliana in complex with 2-carboxyarabinitol-1,5-bisphosphate

The crystal structure of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from Arabidopsis thaliana is reported at 1.5 Å resolution. In light of the importance of A. thaliana as a model organism for understanding higher plant biology, and the pivotal role of Rubisco in photosynthetic carbon assimilation, there has been a notable absence of an A. thaliana Rubisco crystal structure. A. t...

Polypeptide substrate specificity of PsLSMT. A set domain protein methyltransferase.

Rubisco large subunit methyltransferase (PsLSMT) is a SET domain protein responsible for the trimethylation of Lys-14 in the large subunit of Rubisco. The polypeptide substrate specificity determinants for pea Rubisco large subunit methyltransferase were investigated using a fusion protein construct between the first 23 amino acids from the large subunit of Rubisco and human carbonic anhydrase ...

Modified Rubisco Large Subunit N-Methyltransferase Useful for Targeting Molecules to the Active-Site Vicinity of Ribulose-1,5-Bisphosphate

Insight into ribulose 1,5-bis-phosphate carboxylase/oxygenase assembly in maize.

Widely heralded as the most important enzyme on Earth, ribulose 1,5-bis-phosphate carboxylase/oxygenase (Rubisco) catalyzes the reaction that draws inorganic carbon into the biosphere during the Calvin-Benson cycle. In higher plants, algae, andcyanobacteria,Rubiscooccursasahexadecamer consisting of eight large (50 kD) subunits and eight small (13 to 15 kD) subunits. Whereas the large subunits a...